HPLC column (analytical), NUCLEOSIL 100-5 C18 PPN, length: 2

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure Wide acceptance as routine packings for very different fields of chromatography One of the first spherical silicas used in HPLC Developed in the early seventies, it became a world-renowned HPLC packing Still found in many analytical and preparative application An absolutely reliable choice in HPLC The largest variety of modified HPLC silicas available on the market NUCLEOSIL® PPN Silica-based HPLC phases for RP chromatography of biological macromolecules Features Silica-based reversed phase materials with polymerically bonded alkyl chains, exclusively hydrophobic interactions NUCLEOSIL® 100-5 C18 PPN: octadecyl phase · USP L1 Dynamic protein binding capacity per g packing: 8 mg BSA, 64 mg cytochrome C Suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides Particle size 5 µm Pore size 100 Å pH working range 1–9 Max. working pressure 250 bar Eluent in column methanol NUCLEOSIL® 500-5 C18 PPN: octadecyl phase · USP L1 Dynamic protein binding capacity per g packing: 22 mg BSA, 40 mg cytochrome C Especially suited for large peptides and medium-size hydrophilic proteins Particle size 5 µm Pore size 500 Å pH working range 1–9 Max. working pressure 250 bar Eluent in column methanol