Cell Line Origin
Human colorectal adenocarcinoma
Cell Line Description
Genetically modified HRA-19a1.1 cells (Sigma catalog number 09071519) expressing a chimeric alpha2 integrin with the extracellular and transmembrane domain of alpha2 integrin and the cytoplasmic domain of alpha1 integrin.
DNA Profile
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 9,12
THO1: 8
TPOX: 11
vWA: 16
Culture Medium
DMEM (High glucose Sigma D6546) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + G418 200 μg/ml.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:4 weekly using 0.05% trypsin/EDTA; 7.5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Shipping Information:
Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41105500
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- 09071524-1VL
- Temperature Control Device:
- Yes
