Cell Line Origin
Human colorectal adenocarcinoma
Cell Line Description
The HRA-19 cell line was established from a primary human rectal adenocarcinoma present in a 66-year old male. The adenocarcinoma was well differentiated and determined to be Dukes′ stage B. The HRA-19a1.1 cell line is a clone of HRA-19. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.
DNA Profile
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,12
D16S539: 11
D5S818: 11
D7S820: 9,12
THO1: 8
TPOX: 11
vWA: 16
Culture Medium
DMEM - high glucose (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:6 weekly using 0.05% trypsin/EDTA; 7.5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm2.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41106514
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 09071517-1VL
- Temperature Control Device:
- Yes
