Biochem/physiol Actions
HSip-1 DA corresponds to the cell-permeable diacetate ester precursor of the sensitive hydrogen sulfide (H2S)-selective fluorescent turn-on probe HSip-1 (Cyclen-AF-Cu; Hydrogen Sulfide imaging probe-1). Once entering cells, HSip-1 DA is hydrolyzed by esterases to form HSip-1, allowing the selective detection of intracellular H2S by fluorescence (Ex 470-490 nm, Em 510-550 nm). For intracellular loading, incubate cells with 5-30 μM Hsip-1 DA in DMEM at 37 °C for 30 min before removing unincorporated probe by washing with HBSS.
- UPC:
- 51372118
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- SML3403-5MG
