Hsp25 Kinase Inhibitor - Calbiochem (C15-1303-146)

General description

A 13-residue, cell-permeable peptide that acts as a potent and selective inhibitor of mammalian heat-shock protein (Hsp25) kinase [also called mitogen-activated protein kinase-activated protein kinase-2 (MAPKAP kinase-2)]. Inhibition is competitive with respect to the substrate peptide (K_i = 8.1 µM) and non-competitive with respect to ATP (K_i = 134 µM).

A 13-residue, cell-permeable peptide that acts as a potent and selective inhibitor of mammalian heat-shock protein (Hsp25) kinase. Inhibits Hsp25 kinase competitively with respect to the substrate peptide (K_i = 8.1 µM) and non-competitive with respect to ATP (K_i = 134 µM). [Hsp 25 kinase is also called mitogen-activated protein kinase-activated protein kinase-2 (MK2).]

Biochem/physiol Actions

Cell permeable: yes

Primary Target
(Hsp25) kinase

Product does not compete with ATP.

Reversible: no

Target K_i: 8.1 µM against (Hsp25) kinase

Warning

Toxicity: Standard Handling (A)

Sequence

H-Lys-Lys-Lys-Ala-Leu-Asn-Arg-Gln-Leu-Gly-Val-Ala-Ala-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following recosntitution, aliquot and freeze (-20°C). Stock solutions are stable for up to months at -20°C.

Other Notes

Katsoulidis, E., et al. 2005. Cancer Res.65, 9029.
Hayess, K., and Benndorf, R. 1997. Biochem. Pharmacol. 53, 1239.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 1396.68. Empirical Formula: C₆₁H₁₁₃N₂₁O₁₆. Quality Level: 100. Assay: ≥. 98% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic). color: white. solubility: DMSO: soluble, water: soluble. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.