Cell Line Origin
Mouse helper T cell
Cell Line Description
Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).
Application
IL-2 assays
Culture Medium
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g
Other Notes
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biological source: mouse blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Not specified. receptors: Interleukin 2 (IL-2), Interleukin 4 (IL-4). technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92021401-1VL
