Cell Line Origin
HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption
Cell Line Description
HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17
Culture Medium
DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
- UPC:
- 41115404
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 12040401-1VL