General description
Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.
Cell Line Origin
Human hepatocellular carcinoma
Cell Line Description
Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.
Application
Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18
Culture Medium
DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 C. Media change twice a week
Other Notes
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Shipping Information:
Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51201626
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- 01042712-1VL
- Temperature Control Device:
- Yes
