Human Aortic Adventitial Fibroblasts (HAoAF) (C15-1346-021)

General description

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PromoCell Cell Pellets are stored in RNAlater- an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.

Note: RNAlater denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4°C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 μl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.

Cell Line Origin

Adventitial Tissue

Application

Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physical form

Pellet is dissolved in RNAlater^® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subculture Routine

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Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.