Cell Line Origin
Human embryo kidney, infected with endogenous retrovirus of pigs
Cell Line Description
293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.
Application
Pig retrovirus studies, infectivity assays
Culture Medium
DMEM + 2mM Glutamine + 10% HI-FBS
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO2 3
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: human kidney. growth mode: Adherent aggregates. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41202049
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97051411-1VL
