HUMAN GENOMIC DNA

General description

DNA is composed of units called nucleotides arranged into two long polynucleotide chains, resulting in a double helical structure. Nucleotides contain a phosphate group, a sugar group and a nitrogen base. There are four types of nitrogen bases namely adenine (A), thymine (T), guanine (G) and cytosine (C). The arrangement of these bases establishes the genetic codon. The difference in the nucleotide sequences is the reason why organisms differ from one another. In eukaryotic cell, the DNA is localized to the nucleus.

High molecular weight (>50kb) genomic DNA isolated from human blood (buffy coat) by the method of Sambrook et al..

Application

Human Genomic DNA is suitable for:

• Southern hybridization analysis
• genomic library construction
• the amplification of large DNA targets by the Expand System
• to assess the quality or integrity of DNA sample using qPCR or real time (RT) PCR and as a control during DNA sequencing

The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.

Biochem/physiol Actions

DNA is an essential component of the mechanism for heredity. The nucleotide sequences carry information regarding different biological processes. The genetic codons encode proteins essential for biological function. This genetic information is transmitted to the next generation during cell division. The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.

Quality

Molecular weight: The preparation is electrophoretically separated on a 0.5% agarose gel and the gel is stained with ethidium bromide. The molecular weight of the purified genomic DNA is greater than 50kb.
Function test: The preparation is used as template in a PCR with the Expand PCR System and appropriate primers from the human tPA Control Primer Set. Amplification products up to 27kb long are obtained.
Absence of contaminating organisms: The serum used for this preparation was tested for HBs antigen and the presence of antibodies to HIV-1, HIV-2, HCV. All tests were negative.

Physical form

Solution in 10 mM Tris HCl, 1 mM EDTA, pH 8.0

Other Notes

For life science research only. Not for use in diagnostic procedures.