DescriptionAladdin provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE. Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically designed oligo target site (oligo-Tyr). Additionally, Aladdin scientists have demonstrated that Tdp1 will release topo I covalently attached to genomic DNA isolated from cells treated with camptothecin using Aladdin’s In Vivo Link Kit. Tdp1 is thought to be important in repair of topo/DNA adducts that form following topo-active drug therapy; therefore, there is considerable interest in identifying drugs that target Tdp1. Such drugs may work well in combination with topo targeting strategies.Figure 1. SDS-PAGE of Aladdin Purified Tdp1. The gel was intentionally overloaded with 20ug of protein to reveal minor breakdown products.Droduction infoUnit Defintion:One unit of Tdp1 can release the terminal Tyrosine from 20 ng of substrate (oligo-Tyr) in 30 min at 37° C.Tdp1 Reaction:Release of topo I from the covalent complex is shown below.Tdp1 Assays:Biochemical assays for Tdp1 can be carried out using an oligo nucleotide with a 3’ terminal tyrosine residue. Release of tyrosine from a 5’ 32P end labeled oligo is easily measured using a denaturing polyacrylamide gel or DNA sequencing gels. Shipping&storageThis product is shipped on wet ice and should be stored at – 20°C. Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/H489668-SCI_544f1c5506bb9dc8b4380ba6d43f6b76.pdf
- UPC:
- 51311601
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 0.96 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H489668-500U
- Product Size:
- 500U
