Human Umbilical Artery Smooth Muscle Cells: HUASMC, neonatal (C15-1301-841)

General description

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HUASMC has been used to study effects of snake venom on smooth muscle cells (Mora, 2008); secretion mechanism of the (pro)renin receptor (Yoshikawa, 2011) and the role of proteasomes (Martin, 2014) in the development of cardiovascular diseases; and to develop novel material surfaces to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants (Kanie, 2012). Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

vascular function, tissue engineering, pharmacology of vascular tissue

Components

Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HUASMC Culture Protocol.

biological source: human umbilicus (normal arteries). Quality Level: 100. packaging: pkg of 500,000 . cells. manufacturer/tradename: Cell Applications, Inc. growth mode: Adherent. karyotype: 2n : 46. morphology: smooth muscle. technique(s): cell culture | mammalian: suitable. relevant disease(s): diabetes. stroke. cardiovascular diseases. shipped in: dry ice. storage temp.: −. 196°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.