Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
LY9 was produced from a (C57BL/6 x BALB/c)F1 mouse immunised with a single i.p. injection of the lymphoblastoid B-cell line Leakar and B. pertussis adjuvant. The genotype of the B-cell donor for this line was HLA-A2,B13, Cw- DR7, DQ 2/A24,B13, Cw-,DR7 B35, Cw4, DR3, DQ2. Mice spleen was removed 3 days later and fused with the myeloma P3x63Ag8.653. LY9 monoclonal antibodies were shown to react with cells expressing DRw53, DR7 (in the absence of DRw53) and DRw10. LY9 is also known as LK261.
Immunogen
B cell line Leakar HLA A 2,24; B13,35;C4; DR 7,3
Application
Immunofluorescence, cytotoxicity
Culture Medium
RPMI 1640 + 2mM glutamine + 1 mM NaP + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: (C57BL/6 x BALB/c)F1. Myeloma: P3x63Ag8.653. Reactivity: Anti-HLA DR7, DRw10,DRw53. technique(s): cell culture | mammalian: suitable. isotype: IgG3. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41105326
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 99061826-1VL