HYBRIDOMA ANTI-HUMAN CD36 (C15-1314-747)

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.

Immunogen

Human malignant fibrous histiocytoma

Application

Study of endothelial cells, CD36 expression analysis

Culture Medium

RPMI 1640 + 2mM glutamine + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Other Notes

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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen,female. Myeloma: NS-1. Reactivity: Anti-CD36, human. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.