General description
Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
Application
Hydrocortisone is used for the following applications:
- Used in myogenic induction and identification
- Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
- Used in adipogenic differentiation (one of the components in adipogenic medium)
- Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
- The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
- Used in differentiation assays of melanoma spheroid cells
Biochem/physiol Actions
Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
Physical form
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Reconstitution
To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
- UPC:
- 51422415
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H0135-1MG