Hydroxystilbamidine (Fluorogold)

Product introduction：Hydroxystilbamidine (also known as Fluorogold) has been widely used as a retrograde tracer and histochemical stain for neurons. Fluorogold can carry out retrograde axonal transport. It shows a large number of dendrite fillers and has high antifade property.Product parameters：Appearance: yellow solid soluble in water ex/em = 361/536 nmcas No.: 223769-64-0 molecular formula: c18h24n4o7s2 molecular weight: 472.5Matters needing attention：1. for pressure injection by microinjector or micropipette, Fluorogold should be dissolved in distilled water or 0.9% normal saline. Fluorogold can also be used as a suspension in 0.2 M neutral phosphate buffer. However, suspended particles may clog the micropipette tips. For the iontophoresis method, 1% fluorescent gold solution was prepared in 0.1 M acetate buffer (ph=3.3). 2. it can be compatible with almost any fixative or not. The commonly used fixative is 4% paraformaldehyde. Fixatives containing high concentrations of heavy metals (e.g., osmium, mercury) will quench fluorescence, while high concentrations (more than 1%) of glutaraldehyde may increase background fluorescence. 3. Fluorogold containing tissue can be processed according to any common histological technique. These tissues included frozen sections of unfixed tissue (10 μ m) , frozen sections of fixed tissue (20 μ m) , or paraffin sections (3-10 μ m) . 4. injection site: almost any central or peripheral nervous system structure can be injected with Fluorogold to analyze retrograde transport. In the peripheral nervous system, ganglia and peripheral targets can be studied. To study peripheral nerves, nerves should be cut or injured and then immersed or injected with 5% Fluorogold aqueous solution. Since fibers with intact channels will not significantly absorb Fluorogold, fibers must be cut off or severely damaged to absorb dye. 5. fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching. 6. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Cytoplasmic dye, neuronal tracer. Molecular Formula: C18H24N4O7S2. Molecular Weight: 472.50. PubChem CID: 135818273. Isomeric SMILES: CS(=O)(=O)O.CS(=O)(=O)O.C1=CC(=CC=C1/C=C/C2=C(C=C(C=C2)C(=N)N)O)C(=N)N.