Cell Line Origin
Mouse BALB/c Leydig cell testicular tumour
Cell Line Description
Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).
Application
Tumourigenicity studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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biological source: mouse testis. growth mode: Adherent. karyotype: 2n : 40, hyperdiploid. morphology: Epithelial. products: Steroids. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181560
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 89031605-1VL
