Cell Line Origin
Holtzmann rat small bowel adenocarcinoma
Cell Line Description
Derived from X-irradiation induced small bowel adenocarcinoma in adult male Holtzmann rat. Sensitive to lysis by peripheral blood lymphoid cells taken from F344 rats with colon and pancreatic cancers induced by 1, 2 Dimethylhydrazine or 7, 12 DMBT.
Application
Tumourigenicity studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: rat intestine. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51171974
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 88090801-1VL