Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The monoclonal antibody CC101 was produced by inoculating mice with bovine lymphocytes activated with concanavalin A (Con A) The antibody is specific for the WC1 molecule on gamma delta T lymphocytes in cattle that also reacts with porcine, sheep and goat cells. It recognizes a 180,000 MW molecule on the majority of pig CD2- CD4- CD8- T cells. Cells have been grown on macrophage feeder cells and using hypoxanthine and thymidine at concentrations appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Immunogen
bovine Con-A- stimulated lymphoblasts
Application
Flow cytometry, Immunohistology, Immunoprecipitation
Culture Medium
RPMI 1640 + 10% FBS and HT supplement
Subculture Routine
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS-1. Reactivity: Anti-WC1, bovine/porcine. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181708
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 99011338-1VL