Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The hybridoma IAH-CC62 secretes antibodies reactive with bovine CD26 - this was formerly known as WC10. This non-lineage specific antigen is present on bovine leukocytes and has a MW of 115 kDa and 39kDa. Cells have been grown on mouse macrophage feeder cells using hypoxanthine and thymidine at concentractions appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Immunogen
Bovine thymocytes
Application
Immunohistology, Flow cytometry, Immunoprecipation
Culture Medium
RPMI 1640 + 2 mM glutamine + HT supplement + 10% FBS
Subculture Routine
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS-1. Reactivity: Anti-CD26 (formerly WC10), bovine. technique(s): cell culture | mammalian: suitable. isotype: IgG2b. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51201516
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 99011334-1VL
