Cell Line Origin
Frog Grass embryo
Cell Line Description
Previously designated RPH68.2A. Derived from androgenetic haploid embryos of the grass or leopard frog, (Rana pipiens). The cells retain their haploid chromosome complement for over 200 generations. Used in genetic studies.
Application
Genetic studies
Culture Medium
L15 (50%) + Distilled Water (40%) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 25°C. Do not use CO2.Grow cells in dark (i.e wrapped in foil). Cells do not reach 100% confluency, only approximately 70-80%. Freeze in glycerol.
Other Notes
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biological source: frog embryo. growth mode: Adherent. karyotype: 2n : 26, Stable haploid. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 11131607
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 89072615-1VL
