General description
Recombinant, mouse insulin-like growth factor binding protein 3 (IGFBP-3) consisting of amino acids 22-291 and expressed in NS0-derived murine myeloma cells with four substitutions: Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro. Mouse IGFBP-3 cDNA encodes a 291 amino acid residue precursor protein with a putative 27 aa residue signal peptide.
Biochem/physiol Actions
ED₅₀ = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered Acetonitrile, TFA.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS.
Other Notes
Schuller, A.G.P., et al. 1994. Mol. Cell. Endoc.104, 57.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Assay: ≥. 95% (SDS-PAGE). Quality Level: 100. form: solid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. impurities: <. 1.0 . EU/μ. g protein Endotoxin. shipped in: ambient. storage temp.: −. 20°C. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H319. Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313. Hazard Classifications: Eye Irrit. 2. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2.- UPC:
- 12352202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 407254-25UG