Cell Line Origin
BALB/c mouse spleen x Sp2/0
Cell Line Description
IgG-6A6 secretes a mouse monoclonal antibody that binds to the cytoplasmic domain of the very low density lipoprotein (VLDL) receptor. The hybridoma was produced by intraperitoneal injection of a peptide corresponding to the 10 carboxy-terminal amino acids of the VLDL receptor (SVVSTDDDLA) being coupled to keyhole limpet hemocyanin. BALB/c mice spleen cells were then fused with Sp2/0 mouse myeloma for the detection of VLDL receptor in various tissues.
Immunogen
VLDL peptide coupled to keyhole limpet hemocymin
Application
Immunoblot analysis of distribution and regulation of the VLDL receptor in animal tissues and cultured cells.
Culture Medium
DMEM + 2mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C; media renewal every 2-3 days
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: Sp2/0. Reactivity: Anti-VLDL receptor. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51202414
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97040309-1VL
