General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG3 is the has a unique extended hinge region conferring greater flexibility
Human myeloma IgG3, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Application

IgG3, kappa from human myeloma plasma has been used to determine the trisulfide levels in commercial therapeutic IgG1 antibodies and in human myeloma IgG1, IgG2, IgG3 and IgG4 antibodies.

The purified IgG3, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG3 was for in flow cytometry in CHO cells.

Physical form

Solution in 20 mM tris buffered saline, pH 8.0.

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA and SDS-PAGE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.