General description

Identity of IgG3, lambda light chain is verified by subclass monoclonal antibodies and type specific polyclonal antibodies in immunoelectrphoresis and indirect ELISA assays.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG3 is the has a unique extended hinge region conferring greater flexibility
IgG3, λ from human myeloma plasma is purified by fractionation, ion-exchange and affinity chromatography.

Mammalian immunoglobins contain either lambda or kappa light chains.

Application

IgG3, lambda light chain may be used as an antigen for the production and evaluation of antibodies developed for the indentification and classification of IgG3 subtypes. The purified IgG3, lambda may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.

The purified IgG3, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.

Physical form

Solution in 20 mM tris buffered saline, pH 8.0.

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.