Cell Line Origin
Mouse/human hybrid
Cell Line Description
The hybrid cell line II b has been established by transforming mouse NIH 3T3 (ECACC catalogue no. 93061524) with mitotic chromosomes from the human cell line MNNG-HOS (Sigma Catalogue number. 87070201). Various probes showed stable integration of chromosome 7 DNA (7q22-31.3 and 7q22-7 cen) being closely linked to the gene mutation causing cystic fibrosis
Application
Cystic fibrosis studies, diagnostic assays
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Human x Mouse Hybridoma unknown/unspecified. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41181567
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 86072805-1VL
- Temperature Control Device:
- Yes
