IkappaB Kinase Inactive Control Peptide, Cell-Permeable - Calbiochem (C15-1303-257)

General description

An inactive control for IκB Kinase Inhibitor Peptide (Cat. No. 401477). A 14-amino acid peptide corresponding to the mutated recognition sequence of IκB (Ser³²→Ala and Ser³⁶→Ala) fused to the hydrophobic region of the fibroblast growth factor signal peptide to aid in cellular delivery. Does not have any inhibitory effect on LPS-induced IκB degradation by IκB kinases (IKK) in RAW 264.7 cells at 50 µg/ml.

An inactive control for IkB Kinase Inhibitor Peptide (Cat. No. 401477). A 14-amino acid peptide corresponding to the mutated recognition sequence of IkB (Ser³² → Ala and Ser³⁶ → Ala), linked to the hydrophobic region of the fibroblast growth factor signal peptide to aid in cellular delivery. Does not have any inhibitory effect on LPS-induced IκB degradation by IκB kinases (IKK) in RAW 264.7 cells at 50 µg/ml.

Biochem/physiol Actions

Cell permeable: no

Primary Target
An inactive control for IκB Kinase Inhibitor Peptide

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Asp-Arg-His-Asp-Ala-Gly-Leu-Asp-Ala-Met-Lys-Asp-Glu-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Swaroop, N., et al. 2001. Pharm. Res.18, 1631.
Traenckner, E.B., et al. 1995. EMBO J.14, 2876.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 3126.58. Empirical Formula: C₁₃₈H₂₂₉N₃₇O₄₃S. Quality Level: 100. Assay: ≥. 97% (HPLC). form: solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. color: off-white to white. solubility: water: 1 . mg/mL. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.