Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against an antigen present on the surface of reacting bovine T lymphocytes which are CD4- CD8-, gamma/delta TcR+. It immunoprecipitates molecules of 215kDa and 300kDa from resting and activated lymphocytes. The antigen is more prevalent on the lymphocytes of immature animals.
Immunogen
Bovine peripheral blood mononuclear cells
Application
Enumeration of bovine lymphocyte populations
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-WC1, bovine. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51131642
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 91072519-1VL