Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against a polymorphic determinant on bovine IgM. It reacts with serum IgM and IgM+ B cells, precipitating polypeptide chains of 80kDa and 28kDa. It does not react with bovine IgG.
Immunogen
Bovine PBMs, depleted of T lymphocytes
Application
Evaluation of bovine immunoglobulin levels and lymphocyte populations
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-IgM (polymorphic determinant), bovine. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 91072524-1VL