HOT START MASTER MIX

General description

Illustra^™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

Illustra Hot Start Master Mix combines high-quality recombinant Taq DNA Polymerase, a recombinant hot start activator protein, and nucleotides in a proprietary reaction buffer. This ready-to-use mix provides robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success. The mix does not contain Taq antibody and this eliminates the risk of contamination from a mammalian source. Also, since the polymerase is not chemically inactivated, there is no extensive precycling heating step necessary; this reduces the chance of damaging precious DNA samples from heat-induced depurination. The net result from using Illustra Hot Start Master Mix is a more robust and reliable amplification of the target amplicons with increased yield when compared to conventional hot start master mix products.

Features and Benefits

Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.

• Recombinant hot start activator protein sequesters primers prior to PCR, effectively reducing nonspecific priming and primer-dimer formation.
• Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment.
• Avoids the risk of contamination from a mammalian source associated with hot start antibodies.
• Convenient, ready-to-use mix saves time and reduces experimental variability.
• Confidence in downstream applications; increased read length in sequencing applications.

For any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primer-dimer formation.

Packaging

Hot Start Master Mix contains sufficient reagent for 100 PCR reactions in 50 ul final volume (4 x 0.625 ml) and is supplied as a 2x concentrate solution:
20mM Tris-HCl (pH 8.6), 100mM KCl, 3mM MgCl2, 0.4mM dNTPs (dATP, dCTP, dGTP, dTTP), 50 units/ml Taq DNA Polymerase, Hot Start Activator protein, stabilizers.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre, Drive, Forest City, CA 94404, USA. This product is sold under licensing arrangements with Stratagene. The purchase price of this product includes limited, nontransferable rights under U.S. Patent Nos. 5,605,824, 5,646,019, 5,773,257 owned by Stratagene to use only this amount of the product to practice the claims in said patents solely for activities of end users within the field of life science research. Further licensing information may be obtained by contacting the Business Development Department, Stratagene California, 11011 North Torrey Pines Road, La Jolla, California 92307. This product is sold under license from USB Corporation, Cleveland, Ohio, for use as described in the accompanying materials. Further licensing information may be obtained
by contacting USB Corporation, 26111 Miles Road, Cleveland, Ohio 44142. Patent Pending. All rights reserved.

illustra is a trademark of Cytiva