Imidazole buffer Solution (C15-1228-103)

Catalog No.: C15-1228-103
Manufacturer No.: 68268-500ML-F
Manufacturer Name: Sigma-Aldrich
Quantity: 500
Unit of Measure: ML

Price: $603.43

List Price: $670.48

Imidazole is used as a buffer in the pH range of 6.2-7.

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Description
Specifications

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Application

Imidazole buffer solution has been used as a component:

of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
of imidazole wash and elution buffer for protein purification

UPC:: 51282800
Condition:: New
Weight:: 1.00 Ounces
HazmatClass:: No
WeightUOM:: LB
MPN:: 68268-500ML-F

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