Immersion oil (C15-1305-942)

General description

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to approximate the refractive index of glass as closely as possible. It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Principle

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

biological source: synthetic. Quality Level: 100. grade: for microscopy. form: liquid. refractive index: n20/D 1.516. viscosity: 100-120 . mPa.s(20 . °C). density: 1.025 . g/mL . at 20 . °C. application(s): hematology
histology. storage temp.: room temp. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H315 - H319 - H335. Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.