Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose. Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10 15 M -1 ) and streptavidin. Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS PAGE sample loading buffer. Click here to request bulk or custom sizes . Features Strong affinity for avidin, streptavidin and neutravidin Reusable resin, at least 10 times Covalently coupled to limit leaching Spacer arm: 9Å Applications Isolatin of avidin, streptavidin and Neutravidin coupled molecules Immunoprecipitations with avidin, streptavidin and Neutravidin coupled antibodies
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- UPC:
- 12352200
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 786-598
- Temperature Control Device:
- Yes
