IMP-1088 (C09-1139-914)

IMP-1088 is a potent human N-myristoyltransferases NMT1 and NMT2 dual inhibitor with IC 50 s of <1 nM for HsNMT1 and HsNMT2. IMP-1088 has a K d of <210 pM for HsNMT1. IMP-1088 efficiently blocks rhinovirus replication by blocking rhinovirus virus-encoded protein (VP0) N-myristoylation. IMP-1088 protects host cells from the cytotoxic effects of viral infectionIn VitroIMP-1088 (1-1000 nM) prevents virus-induced cytopathic effect (CPE) in a dose-dependent manner with an IC 50 of 17 nM. IMP-1088 (125 nM) complete suppresses of new infectious virus with an IC 50 of 5.8 nM. IMP-1088 (1-1000 nM) blocks production of infectious virus and inhibits single cycle replication in primary human bronchial epithelial cells (hBEC) infected with rhinovirus RV-A1 (MOI 5) for 7 h. IMP-1088 significantly inhibits production of infectious virus even when added up to 3 hours post-infection. IMP-1088 (50 nM) significantly less enriches multiple N-myristoylated proteins in enriched samples. IMP-1088 (500 nM) inhibits production of infectious rhinovirus particles by blocking virus assembly in HeLa cells infected with rhinovirus RV-A16 (MOI 20) for 6 h. IMP-1088 (0.24 nM-1 µM; for 24 hours +24 hours washout) has no long-term effect on cell viability and NMT activity fully recovers by 24 h. IMP-1088 potently blocks a key step in viral capsid assembly by inhibition of co-translational myristoylation of a specific virus-encoded protein (VP0). IMP-1088 blocks neither production of viral RNA, nor translation of rhinovirus polyprotein. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:LiquidIC50& Target:IC50: <1 nM (HsNMT1) and <1 nM (HsNMT2), Kd: <210 pM (HsNMT1). Specifications and Purity: 98%. Molecular Formula: C25H29F2N5O. Molecular Weight: 453.53. PubChem CID: 132274735. Isomeric SMILES: CC1=C(C(=NN1C)C)CCOC2=C(C=CC(=C2F)F)C3=CC4=C(C=C3)N(N=C4CN(C)C)C.