Cell Line Origin
Human Caucasian neuroblastoma
Cell Line Description
Established from an abdominal mass occurring in a 13 month old Caucasian male. The tumour was diagnosed as a neuroblastoma with two rare areas of orgonoid differentiation. The culture is a mixture of two morphologically distinct cell types, a small neuroblast-like cell and the other a large hyaline fibroblast. IMR 32 is partially resistant to polioviruses type 3 and highly resistant to ECHO-II. It is susceptible to Coxsackie B3.
Application
IMR 32 has been used to study the transmigration of cells through a human in vitro blood-cerebrospinal fluid barrier (BCSFB) model.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. The cells have a low plating efficiency and cell attachment can take up to 2-4 days after resuscitation. Cells easily detach. May n
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: human nerve. growth mode: Adherent. karyotype: 2n : 46. morphology: Fibroblast/neuroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41116129
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 86041809-1VL
- Temperature Control Device:
- Yes
