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IN VITRO TOXICOLOGY ASSAY KIT MTT BASED

Catalog No.
C005B-285495
Mfr. No.
TOX1-1KT
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
KT
Price: $971.10
List Price: $1,079.00

General description This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results.

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General description

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Biochem/physiol Actions

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

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UPC:
41151502
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
Yes
MPN:
TOX1-1KT

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