INGEL PEPTIDE DIGESTION, 500 UN1648

A 96-well format kit suitable for processing larger numbers of protein spots concurrently and compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste. Excise protein spots and transfer to the supplied proteomic grade titer plate, destain silver stained gel pieces with SilverOUT™ and then incubate with our proprietary Trypsin-Digestion Buffer Mix, a mixture of MSG-Trypsin™ and an optimal digestion buffer, which ensures reliable and efficient protein digestion. The resulting digested peptides are extracted with Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without the need for further cleaning procedures. Each kit is supplied with: SilverOUT™ destaining reagent. Trypsin Digestion Buffer for optimal trypsin activity. Pep-Extract™ Buffer, for high level peptide extraction. InGel™ Array titer plates and caps The above components are also available individually for added convenience. See the Accessories Section. MSG-Trypsin™, a mass spectrometry grade trypsin with minimal autolysis and increases stability, is available separately, see below. Features Digestion of protein spots in 96 well titer plate format. Supplied with reagents sufficient for up to 500 protein spots. Suitable for 2D gels stained with silver stain, Coomassie dye, and other methods. Applications Digestion of protein spots for mass spectrometry applications. Suitable for 96 well titer plate applications. Suitable for high throughput applications.