General description
A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific, non-competitive and reversible inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine (>Cat. No. 677181)-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
DNA polymerase α and δ
Reversible: yes
Packaging
Packaged under inert gas
Warning
Toxicity: Irritant (B)
Physical form
A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin (Cat. No. 178273) in DMSO.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions.
Other Notes
Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene10, 185.
Urbani, L., et al. 1995. Exp. Cell Res.219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol.46, 1909.Hubermann, J.A. 1981. Cell23, 647.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51452701
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 5047440001