General description
A cell-permeable hydrazone compound that reversibly binds eukaryotic translation initiation factor 4E (eIF4E; KD = 25 µM) and disrupts eIF4E/eIF4G, but not eIF4E/4E-BP1, complex formation. Reported to downregulate the protein, but not mRNA, levels of c-Myc and Bcl-xL in Jurkat cells (8 hrs), leading to apoptotic cell death after prolonged exposure (24 hrs, IC50 ~25 µM). Shown to be brain permeant and reduce neuroligin protein levels, and restore L-LTP threshold and rescue autistic-like behaviours in Eif4ebp2 knockout mice.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
eIF4E
Reversible: yes
Packaging
Packaged under inert gas
Warning
Toxicity: Irritant (B)
Physical form
A 25 mM (5 mg/443 µL) solution of eIF4E/eIF4G Interaction Inhibitor, 4EGI-1 (Cat. No. 324517)
Reconstitution
Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.
Other Notes
Gkogkas, C.G., et al. 2012. Nature493, 371.
Moerke, N. J., et al. 2007. Cell128, 257.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 324519-5MG