General description
A cell-permeable F-actin probe. This macrocyclic cyclodepsipeptide, isolated from the marine sponge Jaspis johnstoni, is a potent inducer of actin polymerization and stabilization in vitro. It competes with Phalloidin (Cat. No. 516640) for actin binding (Kd = 15 nM) and enhances apoptosis induced by interleukin 2 (IL-2) deprivation. Also displays fungicidal, insecticidal, and anticancer properties.
Biochem/physiol Actions
Cell permeable: no
Kd = 15 nM for actin binding
Primary Target
F-actin
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Warning
Toxicity: Harmful (C)
Physical form
A 1 mM (50 µg/71 µl) solution of Jasplakinolide, Jaspis johnstoni (Cat. No. 420107) in DMSO.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Rosado, J.A., et al. 2000. J. Biol. Chem.275, 7527.
Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.
Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.
Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.
Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 709.67. Empirical Formula: C36H45BrN4O6. Quality Level: 100. Assay: ≥. 90% (HPLC). form: liquid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. shipped in: wet ice. storage temp.: −. 20°C.- UPC:
- 51282328
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 420127-50UG
