General description
A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 in H₂O.
Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.
Application
InSolution
Biochem/physiol Actions
Primary Target
ROCK
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Physical form
A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 (Cat. No. 688000) in H₂O.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Other Notes
Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J.351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1999. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science.285, 895.
Uehata, M., et al. 1997. Nature.389, 990.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41161503
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 5092280001