Interferon beta from mouse

General description

IFN β (interferon β) is from the type 1 interferon family of cytokines.

Interferon β (IFN-β) possesses a signal peptide region. It has comparatively more hydrophobic residues and displays 30% homology with IFN-α.

The gene encoding interferon β (Infb1) is localized on mouse chromosome 4.

Application

Interferon β from mouse has been used:

• in the stimulation of bone marrow-derived dendritic cells (BMDCs)
• as a cytokine to stimulate differentiation of podocytes
• to stimulate splenocytes

Interferon ß from mouse has been used:

• to induce the activation of cholesterol 25-hydroxylase, by in vitro stimulation of bone marrow-derived macrophages, dendritic cells and CD11c⁺ lung macrophages
• to stimulate splenocytes, in order to study the secretion and regulation of IFN-γ
• to intranasally pre-treat neonatal mice, to study its effects on the protective actions of Lactobacillus rhamnosus GG (LGG)
• as a standard to treat MEF-Mx2-luc-BKO reporter cell line for IFN-β measurement, in response to human adenovirus-5 (HAdV-C5)

Biochem/physiol Actions

In presence of virus infection, IFN β (interferon β) is upregulated and plays a crucial role in innate antiviral response. It is also important for anti-inflammatory effects.

Interferon β (IFN-β) suppresses a variety of proinflammatory cytokines in lymphocytes including the interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). It also favors the synthesis of antagonists associated with interleukin IL-10 and IL-1 receptor. IFN-β downregulates interferon γ production but enhances transforming growth factor β1 expression. It has therapeutic potential in treating rheumatoid arthritis and relapsing-remitting multiple sclerosis (MS).

Interferon β has a role in the activation of human leukocyte antigen (HLA) gene expression. It acts as a messenger during bacterial infections.

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Physical form

Solution in 20 mM HEPES, pH 6.0, 0.5 M NaCl, 6% glycerol, and 0.1% bovine serum albumin.

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon β.