Interleukin-1 beta; Murine IL-1 beta, 1mg

IL-1 beta, Interleukin-1 beta, murine (mouse): Murine Interleukin-1 beta IL-1β is a pro-inflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Source: Escherichia coli. Molecular Weight: Approximately 17.5kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. Quantity: 2ug/10ug/1000µg Purity: >98% by SDS-PAGE and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.002 ng/ml, corresponding to a specific activity of > 5 x 108 units/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  Formulation: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. AA Sequence: MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS Endotoxin: Less than 1EU/mg of rmIL-1β as determined by LAL method