INTERLEUKIN-10 HUMAN RECOM 1PC X 5UG (C15-1303-322)

General description

Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines.

Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Human IL-10 has cross-species reactivity and is active in mouse cell lines.

Biochem/physiol Actions

ED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.

Warning

Toxicity: Standard Handling (A)

Physical form

Carrier-free.

Reconstitution

Following reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute in sterile water or buffer to a final concentration of 0.1-1 mg/ml as stock solution. Further dilute in buffer such as PBS, 1% BSA.

Other Notes

Bachis, A., et al. 2001. J. Neurosci.21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol.98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Assay: ≥. 95% (SDS-PAGE). Quality Level: 100. form: lyophilized. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. impurities: <. 100 . pg/μ. g Endotoxin (pg/μ. g IL-10). shipped in: ambient. storage temp.: −. 70°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.