Application
Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:
- peripheral blood mononuclear cells (PBMCs) for immunologic assessment
- oocytes for parthenogenetic activation
- T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay, and antigen-specific suppressive function assay
- in combination with phorbol12-myristate 13-acetate (PMA) to stimulate T-cell activation in various cells
- as sperm capacitation medium supplement to study its impact on in vitro fertilization and increasing the efficiency of bovine embryo development
- as a positive control for the detection of intracellular Ca2+ in adipose-derived stem cells (ASCs)(4)
Biochem/physiol Actions
Ca2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca2+; non-fluorescent; used to study Ca2+ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.
Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.
Preparation Note
Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.
- UPC:
- 41116127
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- I0634-1MG
- CAS:
- 56092-82-1
