Iron Oxidizing Medium (Twin Pack), 2.5KG

Iron Oxidizing Medium (Twin pack) is recommended for the isolation, cultivation and enrichment of Thiobacillus ferroxidans. Magnesium sulphate, ammonium sulphate, potassium chloride and calcium nitrate in the medium are sources of ions that stimulate metabolism. Dipotassium phosphate buffers the medium. On preparation, medium is opalescent due to slight precipitate and is green in color. Thiobacillus ferrooxidans grows luxuriantlly on the medium by utilizing ferrous sulphate as energy source. An increase of deep orange brown color as compared to uninoculated controls in positive enrichment tubes or flasks indicates growth. T. ferroxidans can also be enumerated by MPN technique. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis Appearance of Part A: White to cream homogeneous free flowing powder Appearance of Part B: Greenish yellow to dark green homogeneous hygroscopic powder Color and Clarity of Prepared medium: Brownish yellow clear to slightly opalescent with precipitate Reaction: Reaction of (0.39 gm Part A in70 ml distilled water containing 1 ml of 10N sulphuric acid)+ 4.42 gm of Part B in 30 ml distilled water at 25°C. pH : 3.3±0.3 Cultural Response: Cultural characteristics observed after an incubation at 30°C upto 5 days