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iscoveã†s modified dulbeccoã†s medium with sodium bicarbonate and stable glutamine liquid sterile-filtered suitable for cell cultu

ISCOVEÆS MODIFIED DULBECCOÆS MEDIUM WITH SODIUM BICARBONATE AND STABLE GLUTAMINE LIQUID STERILE-FILTERED SUITABLE FOR CELL CULTU

Catalog No.
C15-1425-365
Manufacturer No.
FG0465-500ML
Manufacturer Name
Sigma-Aldrich
Quantity
500
Unit of Measure
ML
Price: $78.22
List Price: $86.91

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture.

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General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

UPC:
51132304
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
FG0465-500ML


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