DescriptionIsocitrate (Isocitric Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.Preparation instructionsSuitable for the quantitative determination of isocitrate and evaluation of drug effects on its metabolism in food, beverage and biological samples (e.g. cell lysate, tissue homogenate, serum, etc.).PrincipleThis Isocitrate Assay Kit measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ex = 530 nm/ em = 585 nm, is proportional. Specification: sufficient for 100fluorometrictests Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/I486409-SCI_f2736dd4d5afd90b15bac5f5ff13c9d4.pdf
- UPC:
- 41142010
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 3.53 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- I486409-1kit
- Product Size:
- 1kit
