ISOLATE II Plasmid Mini Kit (250 preps)

Highly efficient purification of plasmid DNA by combining fast SDS/alkaline lysis of the starting material with the speed and purity of silica-membrane column purification. Product Highlights Fast - streamlined protocol for extraction of eighteen plasmid samples in as little as 15 minutes Efficient – rapid SDS/alkaline lysis and efficient column-binding for high-yield recovery of all plasmid molecules High-performance – efficient extraction of high-purity plasmid DNA, ideal for use in all downstream applications Flexible – reliable recovery of high- and low-copy plasmids from all gram-positive bacteria Product Description The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol. By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria. The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR. Meridian Life Sciences, Inc. products are intended for sale/shipment within the US only. International customers should contact their local Meridian Life Sciences, Inc. distributor to place orders for Meridian Life Sciences, Inc. products.